FIG. 6.

Influence of DEK phosphorylation on DNA binding. (A) Southwestern analysis. DEK was transferred to a nitrocellulose membrane and incubated with radioactively labeled, HinfI-digested SV40 DNA. The autoradiograph (³²P-DNA) and the Western blot (α-DEK) are shown (−P, dephosphorylated DEK; +P_ck2, DEK phosphorylated by CK2 in vitro). (B) Filter binding assay. ³²P-labeled, HinfI-digested SV40 DNA was incubated in the absence (0) or presence of increasing amounts of either dephosphorylated (−P) or phosphorylated (+P_ck2) DEK. The assay was performed with either 50 or 100 mM NaCl. DEK/DNA molar ratios are indicated.