FIG. 2.
Far Western analysis. Twenty nanomoles of full-length DEK (wt, lane 1) and different DEK deletion mutant forms were separated on a 10 to 18% polyacrylamide gel and blotted onto a nitrocellulose membrane. The membrane was then incubated with 32P-labeled full-length DEK. A, Ponceau staining; B, autoradiography. Molecular sizes of marker proteins are given in kilodaltons. (C) DEK protein with the multimerization domain. Dark grey boxes, highly acidic regions; light grey box, SAF-box, a potential DNA binding domain; black box, nuclear localization sequence (NLS). wt, wild type.