Figure 2.

CCl₄-induced liver injury and steatosis were not affected by ethanol feeding to mice. Mice were allowed free-access to a 2% (v/v) ethanol containing diet for two days and then were exposed to CCl₄ and euthanized 24, 48, 72 or 96 h thereafter, while remaining on the ethanol diet. Control animals were pair-fed a diet that isocalorically substituted maltose dextrins for ethanol. (A) Plasma ALT was used to determine hepatic injury; (B) A biochemical assay was used to quantify hepatic triglyceride content. N = 4–8 mice per group.