Figure 5.

Hepatocyte apoptosis was increased in livers from ethanol-fed mice 24 and 48 h after CCl₄ exposure. The terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was used to determine the number of apoptotic hepatocyte nuclei in livers from pair- and ethanol-fed mice 24, 48, and 72 h after CCl₄ exposure. (A) Quantification of the number of TUNEL-positive hepatocyte nuclei in each 200× image (three non-overlapping images per animal); (B) Representative TUNEL staining from pair- and ethanol-fed mice 48 h (maximum hepatocyte apoptosis) after CCl₄ exposure. N = 4 mice per group. * p < 0.05.