Figure 7.

Phosphorylation of the retinoblastoma (Rb) protein was increased by moderate ethanol feeding to mice. Mice on ethanol-containing diets were exposed to CCl₄ and euthanized 24–96 h later. Immunoblots were used to determine hepatic content of phospho-Rb (Ser780) in livers from each mouse. (A) Representative phospho-Rb immunoblot. GAPDH was used as a loading control; (B) Semi-quantification of band densities from all blots after normalization to GAPDH. Data are graphed as fold change over pair-fed, olive oil-treated mice. N = 4–8 mice per group. * p < 0.05.