Figure 10.

Moderate ethanol feeding enhanced hepatic stellate cell (HSC) activation after acute CCl₄ exposure. (A) Real time PCR was used to measure hepatic Acta2 transcript accumulation. Data are expressed as fold change over pair-fed, olive oil-exposed mice after normalization to 18S; (B) Semi-quantification of αSMA band density expressed as fold change of pair-fed, olive oil exposed mice after normalization to GAPDH as a loading control; (C) Representative αSMA immunoblot. Hepatic Col1a1 (D) and Serpinh1 (E) transcript accumulation were determined using real-time PCR. Data are expressed as fold change over pair-fed, olive oil-exposed mice after normalization to 18S. N = 4–8 mice per group. * p < 0.05.