Figure 11.

Matrix metabolism was sustained by ethanol feeding to mice after acute CCl₄ exposure. In situ zymography was used to determine area of gelatinase activity in frozen liver sections from pair- and ethanol-fed mice after CCl₄ exposure. (A) Representative fluorescence microscopy images. The green fluorescence indicates area of gelatinase activity. The blue fluorescence (DAPI) was used as a nuclear counter stain. The asterisks denote location of central veins, while the plus sign (PF, 72 h only) denotes the location of a portal vein. Matrix metabolism occurs most robustly in the pericentral area where CCl₄-induced liver injury and subsequent liver regeneration occurs; (B) Quantification of the area of fluorescence per 200× image as a fold change over pair-fed, olive oil-treated mice. PF = Pair-fed, EF = Ethanol-fed. N = 3–6 mice per group (CCl₄). * (in B), p < 0.05.