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. 2004 Jul;24(13):5821–5834. doi: 10.1128/MCB.24.13.5821-5834.2004

FIG. 2.

FIG. 2.

Interaction of Dyrk1A with Arip4 in HEK293 cells. The indicated proteins were expressed in HEK293 cells. Whole-cell lysates were subjected to immunoprecipitation (IP) with anti-FLAG beads. Immunoprecipitated proteins (left panels) were analyzed by immunoblotting (Western blotting [WB]) with an anti-EGFP antibody for the detection of EGFP-Arip4 and EGFP (upper and lower panels). The membrane then was reprobed with an anti-FLAG antibody for the detection of FLAG-Dyrk1A (middle panel). Note that FLAG-Dyrk1A interacts with EGFP-Arip4 (upper panel, second lane; the lower band is a degradation product of the fusion protein) but not with EGFP alone (lower panel, first lane). Cell lysates were also immunoblotted to detect the expressed proteins (right panels; Input): EGFP-Arip4 (upper panel), FLAG-Dyrk1A (middle panel; arrowheads indicate the fusion protein), and EGFP (lower panel). Protein markers are indicated on the right.