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. 2016 Jan 6;6(1):8. doi: 10.3390/biom6010008

Figure 4.

Figure 4

Immunostaining of Western blots with mAb 114-2G11-A (anti-GlcA) of cell lysates from S2-wt and dGlcAT-P (P) or dGlcAT-S (S) overexpressing cells reveals a strong increase of GlcA-epitopes in cells overexpressing GlcAT-P in the mass range around 60–70 kDa. A silver-stained SDS-PAGE of proteins immunoprecipitated with anti-GlcA specific mAb 144-2G11-A (lane 1) from S2-dGlcAT-P cell lysates (IP-P) shows a comparable pattern with most intense protein staining in the mass range of 60–70 kDa (arrow). Immunoprecipitation with the anti-T antibody HH8 (lane 2) leads to a partial overlap of precipitated proteins, whereas a nonrelevant IgM antibody (lane 3) did not reveal comparable protein enrichment in the respective mass range, indicating that most of the immunoprecipitated protein in lanes 1 and 2 had specifically bound to the carbohydrate-specific antibodies.