Table 1.
Potentially O-glycosylated proteins which were highly modified with GlcA after overexpression of dGlcAT-P in S2-cells. Proteins within a mass range of 60–70 kDa were intensively stained by mAb 114-2G11-A and therefore excised from an SDS-gel and identified by LC-ESI-MS/MS (experiment “gel”) The same lysates was immunoprecipitated with mAb 114-2G11-A and identified likewise (experiment “IP”). (ER: endoplasmatic reticulum, EC: extracellular, N: nucleus, CP: cytoplasm, MI: mitochondrium, MT: microtubule, M: membrane).
| Protein | Identified in Experiment IP (+/−) | Reported Localization | Accession No. (NCBI) |
|---|---|---|---|
| calnexin | + | M, ER | gi|2213427 |
| CD98 heavy chain | + | M | gi|17945866 |
| CG2918, HSP70 family | +/− | EC, M | gi|20128923 |
| ERp60 | +/− | ER | gi|45551086 |
| glycoprotein 93 | +/− | EC | gi|21357739 |
| heat shock protein 60 | +/− | MI | gi|33636453 |
| heat shock protein 83 | +/− | CP | gi|17647529 |
| heat shock protein cognate 1 | − | MT | gi|17647515 |
| heat shock protein cognate 4 | +/− | N, CP, EC | gi|17737967 |
| heat shock protein cognate 71 | +/− | ER | gi|157667 |
| heat shock protein cognate 72 | +/− | ER, EC | gi|157658 |
| Hexosaminidase 2 | + | M, EC | gi|17933586 |
| Inos | +/− | CP | gi|17137626 |
| oligosaccharide transferase | + | M | gi|19922486 |
| peptidase S28 | + | EC, M | gi|20129649 |
| protein disulfide isomerase | +/− | ER, EC | gi|17647799 |
| Scavenger receptor class C | + | M | gi|984515 |
| Ugt58Fa glycosyltransferase | + | M | gi|22024248 |
| Ugt86Da glycosyltransferase | + | M | gi|21357701 |
| vacuolar H[+]-ATPase | + | M, CP | gi|17136796 |
Proteins were identified prior to IP (−), after IP (+) or in both experimental settings (+/−).