FIG. 2.

Other forms of oxidants induced patterns of cell death similar to that of H₂O₂ in RIP^−/−, TRAF2^−/−, and FADD^−/− cells. (A) MEF cells were treated with H₂O₂ (500 μM), tert-butyl hydroperoxide (250 μM), or menadione (10 μM) for 12 h. (B) H₂O₂-induced cell death in Jurkat cells. Both wt and RIP-deficient Jurkat cells were treated with H₂O₂ (500 μM, 12 h) or TNF-α (25 ng/ml plus CHX at 10 μg/ml, 12 h). Cell death was quantified by the determination of LDH leakage. Data are presented as means ± standard deviations from at least three independent experiments.