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. 2004 Jul;24(13):5875–5886. doi: 10.1128/MCB.24.13.5875-5886.2004

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FIG. 3. — The Orc1-associated protein kinase activity is specific to G₂/M phase. (A) Cells were synchronized at the beginning of S phase with aphidicolin and then released. The fractions of cells in G₁, S, and G₂/M phases were determined by FACS analysis. Six examples are shown. (B) Protein kinase activity was assayed in the Orc1 IP from each fraction as described in the legend to Fig. 2. The fraction of cells in G₂/M was calculated from FACS data by using CellQuest software. (C) The fraction of M-phase cells was compared with the ratio of protein kinase activity (intensity of the ³²P-H1 protein band) normalized to histone H1 protein in each fraction. Gel bands were quantified with NIH Image software.