FIG. 9.
Cdk activity does not prevent association of Orc1 with Orc2 during mitosis. (A) Orc1 IPs, Orc2 IPs, and normal IgG IPs were prepared from M-phase and G1-phase cells, as described in the legend to Fig. 4, and the presence of either Orc1 or Orc2 was assayed, as described in the legend to Fig. 5. (B) The experiment described for panel A was repeated except that 1 aliquot of M-phase-arrested cells was treated either with 10 mM 2AP (+ 2AP) or with 30 μM olomoucine (+ Olom) for 2 h before preparation of cell extracts. Cont, control.