FIG. 4.

Hdmx is overexpressed in human primary tumors and amplified in a subset of breast cancers. (a) Correlation between numbers of copies of the Hdmx locus as shown by FISH (x axis) and Hdmx mRNA levels (y axis). (b) The expression of Hdmx mRNA levels was determined by ISH with TMAs. The left panels show a representative portion of ISH for Hdmx with a breast cancer TMA. The bright-field panels show the morphology of tissue samples as revealed by hematoxylin and eosin (H & E) counterstaining. The dark-field panels show ISH analysis of Hdmx expression levels in breast invasive carcinoma and in normal mammary gland. (c) Subset of primary breast tumors showing amplification of Hdmx gene copy numbers as determined by FISH analysis. (Top panel, from left to right) Normal breast tissue sample, fibroadenoma, and carcinoma containing normal copy numbers for the Hdmx gene. (Middle and bottom panels) Representative breast carcinoma tissues showing significant amplification of the Hdmx locus (>6 to 8 copies).