TABLE 1.
Effects of base substitutions in the γF-crystallin promoter on transcription activation by Maf and Sox proteins
| Name | Sequencea | Avg activationb (SD)
|
|||
|---|---|---|---|---|---|
| Maf | Maf + Sox1 | Maf + Sox2 | Maf + Sox3 | ||
| WT | GGCCCCTTTTGTGCTGTTCCTGCCAACACAGCAGACCTC | 3.4 (0.1) | 8.8 (0.6) | 11.6 (0.02) | 16.7 (0.3) |
| M1M2 | GGCCCCTTTTGTGCTGTTCCgcgCAACACAcgcGACCTC> | 1.3 (0.5) | 1.4 (0.1) | 1.9 (0.2) | 2.6 (0.8) |
| M1 | GGCCCCTTTTGTGCTGTTCCgcgCAACACAGCAGACCTC | 1.0 (0.05) | 1.9 (0.05) | 1.7 (0.02) | 4.6 (0.2) |
| M2 | GGCCCCTTTTGTGCTGTTCCTGCCAACACAcgcGACCTC | 1.2 (0.1) | 1.5 (0.02) | 1.9 (0.05) | 3.3 (0.3) |
| M3 | GGCCCCTTTTGTGCTGTTCCTtCCAACACAGCAGACCTC | 1.1 (0.1) | 0.8 (0.06) | 1.7 (0.03) | 1.9 (0.2) |
| M4 | GGCCCCTTTTGTGCTGTTCCTGCCAACACAGaAGACCTC | 1.1 (0.1) | 1.3 (0.01) | 1.6 (0.03) | 1.8 (0.01) |
| M5 | GGCCCCTTTTGgcgTGTTCCTGCCAACACAGCAGACCTC | 4.9 (0.2) | 6.7 (0.4) | 6.7 (0.4) | 15.7 (0.5) |
| S1 | GGCCCCTcagaTGCTGTTCCTGCCAACACAGCAGACCTC | 3.1 (0.3) | 1.5 (0.5) | 1.5 (0.6) | 2.2 (0.4) |
| 1SM | GGCCCCTTTTGTGCgcaTCCTGCCAACACAGCAGACCTC | 3.1 (0.02) | 4.6 (0.4) | 10.8 (1) | 12.4 (0.4) |
| Sp-5 | GGCCCCTTTTGTG GT TGCCAACACAGCAGACCTC | 2.9 (0.3) | 2.1 (0.09) | 2.3 (0.5) | 2.4 (0.02) |
| Sp+2 | GGCCCCTTTTGTGCTGTTCCtgTGCCAACACAGCAGACCTC | 3.3 (0.6) | 2.4 (0.2) | 2.3 (0.1) | 2.6 (0.04) |
| Sp+7 | GGCCCCTTTTGTGCTGTTCCtgccaacTGCCAACACAGCAGACCTC | 2.9 (0.6) | 3.1 (0.2) | 2.7 (0.5) | 3.1 (0.2) |
| Inv | GGCCCCTTTTGTGCTGTTCCTGCtgtgttgGCAGACCTC | 1.3 (0.5) | 1.3 (0.4) | 1.6 (0.3) | 1.7 (0.6) |
| ML | GGCCCCTTTTGTGCTGTgcaTGCCAACACAGCAGACCTC | 1.3 (0.03) | 1.3 (0.01) | 2.3 (0.04) | 1.9 (0.14) |
| MR | GGCCCCTTTTGTGCTGTTCCTGCCAACACAGCAtgcCTC | 3.3 (0.01) | 7.2 (0.01) | 7.1 (0.01) | 11.4 (0.02) |
| MARE | GGCCCCTTTTGTGCTGTTCCTGCtgaCtcaGCAGACCTC | 2.6 (0.9) | 6.4 (0.05) | 8.0 (0.7) | 14.3 (0.14) |
| M-6t | GGCCCCTTTTGTGCTGTTCCTtCtgaCtcaGCAGACCTC | 2.1 (0.07) | 3.2 (0.05) | 4.1 (0.2) | 8.9 (0.01) |
| M+6a | GGCCCCTTTTGTGCTGTTCCTGCtgaCtcaGaAGACCTC | 2.9 (0.01) | 7.5 (0.3) | 8.1 (1.4) | 15.7 (0.3) |
a
Oligonucleotide sequence with the Sox element (double underlined), distal Maf element (single underlined, with dots indicating the nonconsensus core of the Maf recognition sequence), and mutated and inserted bases (lowercase letters) indicated.
b
Activation of the γF-crystallin promoter (CAT activity expressed relative to the activity of the reporter gene cotransfected with a plasmid lacking a transcription factor coding region) by Maf alone or together with Sox1, Sox2, or Sox3. The data represent averages and standard deviations from three or more independent experiments.