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Hypoxia induces telomerase and hTERT expression. (a) JEG-3 cells were cultured at 20% O2 or 1% O2 for 24 h. Whole-cell extracts were prepared and subjected to TRAP assays. (b) JEG-3 cells were cultured at 20% O2 or 1% O2 for 1, 3, 6, 12, and 24 h. Total RNA was prepared and subjected to real-time RT-PCR analysis. (c) JEG-3 and JAR cells were cultured at 20% O2 or 1% O2 for 24 h. Whole-cell extracts (40 μg) were prepared and subjected to Western blot analysis.
