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. 2004 Jul;24(13):6076–6083. doi: 10.1128/MCB.24.13.6076-6083.2004

FIG. 3.

FIG. 3.

Transactivation of hTERT promoter activity by hypoxia exposure and HIF-1 overexpression. (a) Schematic representations of the HIF-1 response elements and the pGL181-luc, pGL181mt1-luc, pGL181mt2-luc, and pGL181mt-luc reporters are shown. (b) JEG-3 cells were cotransfected with the pGL181-luc, pGL181mt1-luc, pGL181mt2-luc, and pGL181mt-luc (0.3 μg each) constructs and 1.0 μg (each) of the HIF-1 expression constructs (HIF-1α and ARNT) or the empty vector pcDNA3 under hypoxic conditions (1% O2); activity is reported as relative luminescence units (RLU). Error bars indicate standard deviations in triplicate assays.