FIG. 9.

(A) COS-7 cells were transfected with FynY531F, which is constitutively active because of a point mutation at the regulatory tail tyrosine (Y531). Cells were either treated with GA only or were pretreated with PP2 for 1 h followed by incubation with both PP2 and GA for 0, 1, 2, or 3 h. Cells were lysed and immunoblotted for Fyn. (B) Cells were transfected with a constitutively active form of c-Src, SrcY527F, and were treated as described for panel A. Cells were lysed and immunoblotted for Src.