FIG. 2.

Time course of TR4 expression during testis development. (A) Total RNA was isolated from testes of mice at different postnatal days as indicated. RT-PCR was performed, with β-actin as an internal control. (B) Total RNA was isolated from testes of mice at different postnatal days as indicated, and real-time quantitative RT-PCR was performed. Results are means ± standard deviations from two or three RNA samples from two or three different mice. All sets of results show the same trend, and one set of data is shown. (C) Timetable of first wave of spermatogenesis, including spermatogonia (Sg), the prelepotene (PL), lepotene (L), zygotene (Zs), pachytene, and diplotene (Ds) stages of germ cell differentiation.