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. 2004 Jul;24(13):5887–5899. doi: 10.1128/MCB.24.13.5887-5899.2004

FIG. 3.

FIG. 3.

Confirmation of knockout of TR4 gene in TR4−/− mice. (A) PCR analysis of mouse genomic DNA. The wild-type and target alleles give 455- and 760-bp PCR products, respectively. (B) RT-PCR analysis of TR4 in TR4+/+, TR4+/−, and TR4−/− mouse testes. Total RNAs from TR4+/+, TR4+/−, and TR4−/− mice were extracted, and RT-PCR was performed. (C) Southern blot analyses of mouse testis DNA from TR4+/+, TR4+/−, and TR4−/− mice. DNA was digested with EcoRI and hybridized with the probe indicated. The expected fragments after EcoRI digestion are 8 kb for the wild-type allele and 4.9 kb for the mutant allele.