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. Author manuscript; available in PMC: 2016 Mar 28.
Published in final edited form as: J Neurochem. 2015 Aug 31;135(2):309–322. doi: 10.1111/jnc.13250

Figure 3. Blockade of Erk1/2 in neurons negates the IFNγ-mediated protection against Stat1-mediated cytotoxicity.

Figure 3

Primary wildtype (A) and Stat1-KO (B) hippocampal neurons were pre-treated with inhibitors FR180204 (Erk1/2 inhibitor), U1026 (Mek1/2 inhibitor), LY294002 (PI3-Kinase inhibitor), and SB190292 (p38-Mapk inhibitor) (10μM). After 1h, IFNγ (100 U/ml) was applied to the cultures in the presence of the inhibitors and incubated for 72h. Cultures were assayed by MTS assay as a measure of cell survival. Statistical analysis was applied by paired t-test (* p<0.05 vs. corresponding untreated; # p<0.01 vs. corresponding untreated; n=2).