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. Author manuscript; available in PMC: 2016 Mar 28.
Published in final edited form as: J Neurochem. 2015 Aug 31;135(2):309–322. doi: 10.1111/jnc.13250

Figure 4. IFNγ activates Erk1/2 in primary neurons but not in fibroblasts.

Figure 4

(A) and (B) Hippocampal neurons and fibroblasts (MEFs) explanted from mouse embryos were treated with IFNγ (100 U/ml). Cells were lysed at indicated times post treatment and subjected to Western blot for Erk1/2, phosphorylated Erk1/2 (Erk1/2-P), and GAPDH as a loading control. C. Western blots from A and B were quantified by densitometry using ImageJ software. Erk1/2 and p-Erk1/2 signal was normalized to GAPDH as a loading control. Statistical analysis was determined using two-way ANOVA (n = 3, p < 0.002).