Table 1.
mGlu3 NAM and mGlu5 SAR of Western Phenyl Ethers 18, 22–39
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no. | R | mGlu3 pIC50 (± SEM)a | mGlu3 IC50 (nM)a | % Glu Max (± SEM)a,b | mGlu5 activityc | mGlu5 pEC50 (± SEM)c | mGlu5 EC50 (nM)c | % Glu Max (± SEM)b,c |
18 | H | 6.57 ± 0.15 | 267 | 2.21 ± 0.80 | PAM | 7.34 ± 0.02 | 46 | 87.7 ± 1.4 |
22 | 2-F | 6.28 ± 0.20 | 530 | 2.90 ± 1.05 | PAM | 6.71 ± 0.04 | 195 | 89.3 ± 3.0 |
23 | 3-F | 6.11 ± 0.03 | 773 | 1.19 ± 0.75 | PAM | 7.14 ± 0.02 | 73 | 86.1 ± 2.9 |
24 | 4-F | 6.34 ± 0.04 | 462 | 2.09 ± 0.57 | PAM | 7.03 ± 0.05 | 92 | 87.0 ± 1.0 |
25 | 2,4-di-F | 6.38 ± 0.22 | 417 | 1.70 ± 0.35 | PAM | 6.51 ± 0.05 | 307 | 82.3 ± 2.7 |
26 | 2-Me | 6.14 ± 0.14 | 721 | 1.56 ± 0.37 | PAM | 5.83 ± 0.04 | 1500 | 83.7 ± 2.5 |
27 | 3-Me | 5.84 ± 0.05 | 1440 | 2.35 ± 0.18 | NAMd | <5.0 | >10000 | 39.7 ± 7.7 |
28 | 4-Me | 5.92 ± 0.02 | 1190 | 1.72 ± 0.55 | PAM | 5.97 ± 0.04 | 1080 | 92.5 ± 2.6 |
29 | 2-Cl | 6.27 ± 0.18 | 532 | 1.63 ± 0.33 | PAM | 6.38 ± 0.01 | 419 | 84.4 ± 2.1 |
30 | 3-Cl | 5.79 ± 0.02 | 1620 | 1.96 ± 0.11 | NAMd | <5.0 | >10000 | 32.8 ± 6.1 |
31 | 4-Cl | 5.93 ± 0.01 | 1170 | 1.86 ± 0.52 | PAM | 6.22 ± 0.04 | 604 | 81.8 ± 0.3 |
32 | 2-OMe | 5.59 ± 0.18 | 2540 | 2.32 ± 1.39 | PAMd | <5.0 | >10000 | 63.7 ± 3.1 |
33 | 3-OMe | 5.89 ± 0.02 | 1290 | 1.78 ± 0.53 | PAM | 5.74 ± 0.04 | 1840 | 32.4 ± 3.0 |
34 | 4-OMe | 5.98 ± 0.02 | 1040 | 1.76 ± 0.39 | PAM | 5.87 ± 0.16 | 1340 | 40.2 ± 4.8 |
35 | 4-CF3 | 5.73 ± 0.01 | 1850 | 4.13 ± 0.09 | <4.5 | >30000 | ||
36 | 4-Et | 6.08 ± 0.05 | 830 | 2.29 ± 0.84 | PAM | 5.49 ± 0.03 | 3210 | 50.8 ± 3.7 |
37 | 4-CN | 6.11 ± 0.12 | 784 | 2.53 ± 0.70 | PAM | 5.71 ± 0.06 | 1940 | 38.6 ± 4.8 |
38 | 4-OEt | 5.94 ± 0.06 | 1160 | 3.39 ± 0.87 | <4.5 | >30000 | ||
39 | 4-OCF3 | 5.39 ± 0.04 | 4070 | 2.66 ± 0.34 | <4.5 | >30000 |
Calcium mobilization mGlu3 assay; values are average of n ≥ 3.
Amplitude of response in the presence of 30 μM test compound as a percentage of maximal response (100 μM glutamate); average of n ≥ 3.
Calcium mobilization mGlu5 assay; values are average of n ≥ 3.
Weak activity; concentration response curve (CRC) does not plateau.