Table 2.
mGlu3 NAM and mGlu5 SAR of Western 2-Pyridyl Ethers 41–51
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no. | R | mGlu3 pIC50 (± SEM)a | mGlu3 IC50 (nM)a | % Glu Max (± SEM)a,b | mGlu5 activityc | mGlu5 pEC50 (± SEM)c | mGlu5 EC50 (nM)c | % Glu Max (± SEM)b,c |
41 | 3-F | 5.96 ± 0.02 | 1100 | 2.22 ± 0.84 | PAM | 5.37 ± 0.06 | 4300 | 78.5 ± 6.1 |
42 | 3-CF3 | 5.53 ± 0.02 | 2940 | 2.11 ± 0.96 | <4.5 | >30000 | ||
43 | 3-OMe | 5.17 ± 0.02 | 6760 | –1.14 ± 2.54 | <4.5 | >30000 | ||
44 | 4-Me | 5.68 ± 0.01 | 2090 | 0.86 ± 0.47 | NAMd | <5.0 | >10000 | 46.9 ± 5.9 |
45 | 4-CF3 | 5.78 ± 0.01 | 1670 | 1.62 ± 0.42 | NAMd | <5.0 | >10000 | 12.9 ± 2.9 |
46 | 4-OMe | 5.43 ± 0.00 | 3690 | –0.18 ± 1.39 | NAMd | <5.0 | >10000 | 54.7 ± 5.8 |
47 | 5-F | 6.27 ± 0.02 | 539 | 1.71 ± 0.55 | NAMd | <5.0 | >10000 | 46.5 ± 3.4 |
48 | 5-Cl | 6.22 ± 0.04 | 605 | 1.84 ± 0.51 | PAM | 5.53 ± 0.06 | 2920 | 81.4 ± 2.7 |
49 | 6-Me | 5.62 ± 0.02 | 2370 | 1.12 ± 1.08 | NAM | 5.65 ± 0.07 | 2250 | 2.47 ± 0.17 |
50 | 6-CF3 | 5.63 ± 0.02 | 2360 | 2.06 ± 0.79 | <4.5 | >30000 | ||
51 | 6-OMe | 5.80 ± 0.02 | 1590 | 1.94 ± 0.62 | PAMd | <5.0 | >10000 | 31.2 ± 3.1 |
Calcium mobilization mGlu3 assay; values are average of n ≥ 3.
Amplitude of response in the presence of 30 μM test compound as a percentage of maximal response (100 μM glutamate); average of n ≥ 3.
Calcium mobilization mGlu5 assay; values are average of n ≥ 3.
Weak activity; CRC does not plateau.