Fig. 3.

The effects of nitric oxide (NO) on jasmonic acid (JA) synthesis. (A) Expression level by quantitative (q)RT–PCR of the two JA biosynthetic genes [LIPOXYGENASE2 (LOX2) and ALLENE OXIDE SYNTHASE (AOS)] at days 3 and 7 in the induction medium of somatic embryogenesis. WT and pgb2 tissue was cultured in medium with altered levels of NO and JA using the pharmacological treatments described in Fig. 2. Values are means ±SE of at least three biological replicates and normalized to the value of the WT (C) of day 3 set at 1. Asterisks indicate statistically significant differences (P≤0.005) from the WT (C) value of the respective day in culture. (B) Number of somatic embryos generated from WT and aos plants. Explants were cultured on medium with altered levels of NO and JA. Values are means ±SE of at least three biological replicates. An asterisk indicates statistically significant differences (P≤0.005) from the WT (C) value set at 100%. (C) Number of somatic embryos generated from WT, pgb2 aos, and pgb2/aos double mutant plants. Explants were cultured on medium with altered levels of NO and JA. Values are means ±SE of at least three biological replicates. An asterisk indicates statistically significant differences (P≤0.005) from the WT value set at 100%.