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. 2016 Mar 9;67(8):2231–2246. doi: 10.1093/jxb/erw022

Fig. 5.

Fig. 5.

Nitric oxide (NO) and jasmonic acid (JA) affect MYC2. (A) Expression level by quantitative (q)RT–PCR of MYC2 at days 3 and 7 in the medium with altered levels of NO and JA using the pharmacological treatments described in Fig. 2. Values are means ±SE of at least three biological replicates and are normalized to the value of the WT (C) of day 3 set at 1. An asterisk indicates statistically significant differences (P≤0.005) from the WT (C) value of the respective day in culture. (B) Expression level by qRT–PCR of MYC2 in WT and aos lines at days 3 and 7 on induction medium. Values are means ±SE of at least three biological replicates and are normalized to the value of the WT at day 3 set at 1. An asterisk indicates statistically significant differences (P≤0.005) from the WT value of the respective day in culture. (C) Number of somatic embryos generated from WT, 35S:MYC2, and myc2-1 plants. Explants were cultured on media with altered levels of NO and JA. Values are means ±SE of at least three biological replicates. An asterisk indicates statistically significant differences (P≤0.005) from the WT value set at 100%.