Fig. 8.

The effect of TK VI on outward K⁺ currents as analyzed by patch-clamp whole-cell recordings. (A) Patch-clamp whole-cell recordings of outward K⁺ current traces in wild-type (WT) and tkr1 root cell protoplasts without (Control) or with 3 μM TK VI. Time and current scales shown in the middle apply to all traces. (B) The current/voltage relationship of the whole-cell outward K⁺ currents as illustrated in (A). The number of protoplasts measured for the curves were 16 (for WT), 14 (for WT treated with 3 μM TK VI), 11 (for tkr1), and 11 (for tkr1 treated with 3 μM TK VI). Data shown are averages with the SD. Samples with different letters are significantly different; P<0.001. (C) Effect of TK VI on auxin distribution gradients in the WT and tkr1. DR5 _pro :GUS/WT and DR5 _pro :GUS/tkr1 seeds were germinated on medium without (MS) or with 3 μM TK VI for 5 DAG before GUS staining assays were performed. Scale bars=50 µm.