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. 2016 Feb 17;67(8):2263–2275. doi: 10.1093/jxb/erw032

Fig. 5.

Fig. 5.

In vitro and in vivo interaction between MaDof23 and MaERF9. (A) A Y2H assay for the interaction between MaDof23 and MaERF9. The coding regions of MaDof10, 23, 24, and 25-N were fused with pGBKT7 (BD) and the coding region of MaERF9 with pGADT7 (AD) vectors as indicated, and co-transformed into the yeast strain Gold Y2H. The ability of yeast cells to grow on QDO medium (SD/−Leu−Trp−Ade−His but containing 125 μm aureobasidin A), and to turn blue in QDO medium containing 4mg mL−1 X-α-Gal, was scored as a positive interaction. (B) BiFC in tobacco leaf epidermal cells showing the interaction between MaDof23 and MaERF9 in living cells. MaDof23 and MaERF9 were fused with the N-terminus of YFP (YNE) or the C-terminus of YFP (YCE), as indicated, and co-transfected into N. benthamiana leaves by A. tumefaciens infiltration. Expressions of MaDof23 or MaERF9 alone were used as negative controls. YFP indicates fluorescence of YFP; Merge indicates a digital merge of bright field and fluorescent images. Scale bar, 30 μm.