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. 2016 Feb 19;67(8):2367–2386. doi: 10.1093/jxb/erw040

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© The Author 2016. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 11. — JAZ7 contains an active EAR-repression motif mediating its interaction with TPL, and interacts in vitro with MYC3, MYC4 and JAM1. (A) JAZ7 repression domain sequence used to generate a Cup-Shaped Cotyledon (CUC) repression construct. The EAR motif is red underlined. (B) The chimeric protein in which CUC1 was fused to the JAZ7^EAR domain was expressed in transgenic plants (35S:CUC1-JAZ7 ^EAR). Representative wild-type (WT) and 35S:CUC1-JAZ7 ^EAR seedlings are shown. (C) Y2H assays between Topless (TPL), MYC2, MYC3, MYC4 or JAM1 and JAZ7, a version of JAZ7 containing a mutated EAR-motif (JAZ7mEAR), JAZ5 or JAZ8. Y2Hs were performed on non-selective SD-Leu-Trp media as well as selective SD-Leu-Trp-His or SD-Leu-Trp-His-Ade media. No interactions were seen for the binding domain (BD) only or when an empty activation domain (AD) was used on selective media. JAZ8 and JAZ5 were used as positive controls for TPL interactions. Results were replicated in several independent experiments.