Fig. 12.

JAZ7 binds TPL in vivo. Co-immunoprecipitation analysis from transiently expressed proteins in N. benthamiana leaves showing association between TPL and JAZ7, JAZ8 or JAZ5 but not JAZ7mEAR. TPL was fused to C-terminal Myc tag while JAZ7, JAZ5, JAZ8 and JAZ7mEAR are fused to C-terminal YFP tag. Immunoblots show the presence of proteins in total extracts (input, top panels) and after immunoprecipitation with anti-GFP beads (IP-GFP, bottom panels). JAZ8 and JAZ5 were used as positive controls and GFP vector as a background control. Protein molecular mass ladder is shown to the right of each blot. Results were replicated in an independent experiment. Asterisks indicate the expected protein bands.