Table 1.
Kinetic parameters of recombinant CsUGT78A14 and CsUGT78A15 proteins
| Enzyme | Substrate | K m (μM) | V max (pKat·mg −1) | K cat (S −1) | K cat /K m (S −1 ·M −1) |
|---|---|---|---|---|---|
| CsUGT78A14 | GlcT activity | ||||
| Kaempferol | 30.9±3.5 | 73.6 | 6.75±0.03 | 0.219 | |
| Quercetin | 11.9±1.6 | 36.2 | 3.32±0.07 | 0.280 | |
| Myricetin | 23.8±2.1 | 33.9 | 3.11±0.04 | 0.131 | |
| UDP-Glc* | 14.1±1.8 | 39.3 | 3.61±0.02 | 0.257 | |
| CsUGT78A15 | GalT activity | ||||
| Kaempferol | 54.6±4.5 | 265.1 | 24.49±0.83 | 0.448 | |
| Quercetin | 50±5.2 | 183.5 | 16.95±0.50 | 0.339 | |
| Myricetin | 38.8±2.3 | 115.2 | 1.06±0.12 | 0.027 | |
| UDP-Gal* | 43.3±6.5 | 150.5 | 13.90±0.95 | 0.321 | |