Fig. 3.

Cell death and expression of immunity- or thermotolerance-related marker genes were triggered by transient overexpression of 35S::CabZIP63. (A) Cell death triggered by transient overexpression of 35S::CabZIP63, displayed with phenotype, DAB staining, and trypan blue staining at 4 dpi, respectively. (B) Quantification of electrolyte leakage as ion conductivity to assess the cell death response in leaf discs. (C) Quantitative real-time RT–PCR analysis of the expression of immunity- or thermotolerance associated marker genes in 35S::CabZIP63 and 35S::CabZIP63-SRDX expressed in pepper leaves at 24 hpi, respectively. Data represent the means ±SD from four independent biological replicates. Different letters above the bars indicate significantly different means (P<0.01), as analyzed by Fisher’s protected LSD test. (This figure is available in colour at JXB online.)