Fig. 3.

The aln-1 mutant enhances wounding-inducible expression of genes involved in JA biosynthesis and signaling. RNA was extracted from aerial parts of 2-week-old seedlings of the WT, aln-1 mutant, and the complemented mutant (aln-1 35S:ALN) at the indicated time points after wounding of rosette leaves. Relative mRNA levels were determined as described in Fig. 1C and are presented as values relative to those of the WT at zero time. Data are means ±SE from three independent experiments, and asterisks denote significant differences between WT and mutant plants (*P<0.05; **P<0.01 by Student’s t-test).