Fig. 5.

Exogenous allantoin induces JA-responsive gene expression in an ABA-dependent manner. (A) Effects of exogenous allantoin and allantoate on JA-responsive gene expression in the WT. RNA was extracted from aerial parts of sterile seedlings that had been grown for 2 weeks on standard medium containing allantoin or allantoate at 100 µM, and relative mRNA levels for each gene were determined as described in Fig. 1C. Data are means ±SE from three independent experiments, and asterisks denote significant differences between control and treated seedlings (*P<0.01; **P<0.001 by Student’s t-test). (B and C) Effect of exogenous allantoin on JA-responsive gene expression in bioactive JA-deficient jar1-1 and JA-insensitive myc2-3 mutants (B), and in ABA-deficient aba2-1 and bglu18 mutants (C). WT data are the same as presented in (A) and are shown for comparison. Data are means ±SE from three independent experiments, and different letters indicate significant differences (P<0.05 by Tukey’s test).