Fig. 2.

PCFT expression in primary NS-NSCLC and normal lung specimens. (A) Results for quantitative real-time RT-PCR are shown for 26 NS-NSCLC and 8 normal lung specimens (Origene). PCFT transcript levels were normalized to transcript levels for β-actin. The median value for the normal lung specimens was assigned a value of 1. (B and C) IHC results are shown for 61 NS-NSCLC and 10 normal lung tissues from a tissue microarray (TMA) (US Biomax, Inc.). The TMA was incubated with affinity-purified PCFT-specific antibody or rabbit IgG, and the slides were developed, counterstained, and mounted, as described in Materials and Methods. Representative images are shown in (C) for IgG and for three NS-NSCLC specimens with low-, intermediate-, and high-level staining (left to right). The slides were scanned by an Aperio Image Scanner (Aperio Technologies, Inc.) for microarray image scanning. The total positive cell numbers and intensity of antibody staining of each tissue core were computed and are shown in (B). The median value for the normal lung specimens was assigned a value of 1. Statistical significance between the groups was analyzed by the student’s t test. An asterisk indicates a statistically significant difference between the median NS-NSCLC value and the median value for the normal lung specimens (P < 0.001).