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. 2016 Apr;89(4):457–466. doi: 10.1124/mol.115.101808

Fig. 1.

Fig. 1.

(A) Scheme of the CAMYEL sensor configuration where the intramolecular conformational change triggered by cAMP results in a decrease in BRET due to RLuc8 and YFP changing their relative orientation from each other. (B–G) Dose–response experiments of adenylyl cyclase inhibition by dopamine (●) or norepinephrine (○) mediated by D2LR, D2SR, D3R, D4.2R, D4.4R, and D4.7R in HEK-293T cells transiently expressing the CAMYEL sensor and the indicated D2-like receptor: (B) D2LR, (C) D2SR, (D) D3R, (E) D4.2R, (F) D4.4R, or (G) D4.7R. Cells were treated with forskolin (10 µM) for 10 minutes followed by dopamine or norepinephrine and BRET values obtained by forskolin alone were subtracted from BRET values for each agonist concentration. Data represent the mean ± S.E.M. of 6 to 11 experiments performed in triplicate and are shown as a percentage of the maximal effect (see Table 2 for EC50 and Emax values and statistical comparisons).