. 2016 Apr;89(4):457–466. doi: 10.1124/mol.115.101808

TABLE 2.

Adenylyl cyclase inhibition by dopamine and norepinephrine mediated by D2LR, D2SR, D3R, D4.2R, D4.4R, and D4.7R

Results are from adenylyl cyclase inhibition-BRET experiments in HEK-293T cells transiently transfected with the CAMYEL sensor and one of the indicated receptors. Data were fit by nonlinear regression to a sigmoidal dose–response relationship against the agonist concentration. The EC₅₀ and E_max values are mean ± S.E.M. of 5 to 11 experiments performed in triplicate. E_max values are expressed as 1000 × [BRET_max − BRET_basal].

Receptor	Dopamine		Norepinephrine		NE/DA (EC₅₀)
Receptor	EC₅₀ (nM)	E_max	EC₅₀ (nM)	E_max	NE/DA (EC₅₀)
D2LR	9.4 ± 1.1	94 ± 8	88 ± 17	94 ± 6	9.4
D2SR	6.7 ± 0.5^a	140 ± 7^a	62 ± 6	149 ± 8^a	9.3
D3R	1.6 ± 0.2^a	48 ± 7^a	19 ± 3^a	50 ± 5^a	12
D4.2R	1.8 ± 0.2^a	74 ± 8	54 ± 8	69 ± 8	30
D4.4R	1.4 ± 0.2^a	98 ± 9	53 ± 8	79 ± 6	38
D4.7R	1.6 ± 0.3^a	83 ± 11	53 ± 13	84 ± 6	33

NE/DA, ratio between EC₅₀ values of norepinephrine and dopamine for each receptor.

^{^a}

P < 0.001, significantly statistically different compared with D2LR (one-way analysis of variance, followed by post hoc Newman-Keuls test).