TABLE 2.

Kinetic parameters of glyburide transport in HEK cells overexpressing BCRP protein (WT or C421A)^a

	Genistein (µM)
	0	2	10
WT-BCRP
Km (µM)	70.5 ± 17.0	12.1 ± 6.59b	1.0 ± 0.19b
Jmax (pmol 3H-glyburide/mg protein)	0.69 ± 0.17	0.68 ± 0.12	0.89 ± 0.23
C421A-BCRP
Km (µM)	14.5 ± 1.48c	2.52 ± 1.84b	0.61 ± 0.22b
Jmax (pmol 3H-glyburide/mg protein)	0.73 ± 0.21	0.79 ± 0.11	0.82 ± 0.14

^aK_m and J_max values were calculated using Graphpad nonlinear regression analysis (Michaelis-Menten) and are presented as mean ± S.D. (n = 3 independent experiments).

^bP < 0.05 compared with 0 µM genotype control.

^cP < 0.05 compared with 0 µM WT-BCRP control.