Fig. 5.

Inhibition of superoxide dismutase as an indication of oxidative stress. Copper/zinc SOD was incubated in the absence or presence of increasing concentrations of DCF (□) or DCF-AG (▪) for 30 minutes at room temperature (∼25°C). Vehicle shows assay performance in the presence of the same level of organic solvent (dimethyl sulfoxide) used in the inhibitor incubations. Each bar reflects the mean ± standard error of the mean from three separate studies (n = 2 replicates per study). **P < 0.01, ***P < 0.001 inhibitor versus its vehicle incubation. ^#P < 0.05, ^###P < 0.001 for DCF versus DCF-AG.