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. 2016 Mar 22;11:1147–1158. doi: 10.2147/IJN.S100156

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© 2016 Liu et al. This work is published and licensed by Dove Medical Press Limited

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(A) ARS staining of MC3T3-E1 cells cultured for 21 days in osteogenic induction microenvironments containing different released mediums. (B) Quantification of mineral deposition by detecting absorbance of ARS extracts.

Notes: Scale bar =200 μm. Error bars represent SD. ^*P<0.05.

Abbreviations: BMP-2, bone morphogenetic protein; PlGF-2, placental growth factor-2; GF, growth factor; OD, optical density; ARS, Alizarin Red S; SD, standard deviation.