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. Author manuscript; available in PMC: 2016 Mar 28.
Published in final edited form as: J Neuroendocrinol. 2015 Jul;27(7):624–635. doi: 10.1111/jne.12293

Figure 2.

Figure 2

Synaptic inputs to kisspeptin neurones in the POA and ARC. A: Confocal images (1 μm thickness; 63×) of triple-labeling for kisspeptin (green), vGlut2 (red) and synaptophysin (blue) in the ARC of a luteal phase ewe. White arrows indicate examples of a triple-labelled kisspeptin/vGlut2/synaptophysin terminal in close contact with a kisspeptin soma, and red arrows indicate an example of a dual-labeled vGlut2/synaptophysin terminal. Scale bar, 10 μm. B: Total number of synaptophysin-positive contacts onto kisspeptin neurones in luteal (n=4) and surge (n=4) animals. No differences were seen between groups in the total number of synaptic inputs to POA kisspeptin neurones, but ARC kisspeptin neurones received significantly more synaptic inputs in surge animals. *, P < 0.05. Data presented as means ± SEM.