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. Author manuscript; available in PMC: 2016 Mar 28.
Published in final edited form as: J Neuroendocrinol. 2015 Jul;27(7):624–635. doi: 10.1111/jne.12293

Figure 3.

Figure 3

Kisspeptin- and vGlut2-positive inputs onto GnRH neurones located in the POA and MBH. A: Confocal images (1 μm thickness; 63×) triple-labelled for kisspeptin (green), vGlut2 (red) and GnRH (blue) in the POA and MBH. White arrows indicate examples of dual labelled kisspeptin/vGlut2 terminal in close contact with a GnRH soma. Yellow arrow indicates an example of a single-labeled kiss input to a GnRH neurone in the POA. Scale bar, 10 μm. B: Total number of contacts onto GnRH neurones from kisspeptin and vGlut2 labelled terminals (single and dual-labelled). GnRH neurones in the MBH receive significantly more kisspeptin and vGlut2 inputs during the surge than the luteal phase. *, P < 0.05. Data presented as means ± SEM.