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. 2015 Oct 13;1:15028. doi: 10.1038/celldisc.2015.28

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Copyright © 2015 SIBS, CAS

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Both ubiquitin-specific protease 22 (USP22) and cyclin B1 (CCNB1) are upregulated in human colon cancers. (a) Flag-CCNB1 pull-down products from affinity purification using HCT116 cells were separated by SDS–PAGE and visualized by coomassie brilliant blue staining. (b) Interaction network of CCNB1-associated proteins. (c) The lysates of frozen colon tumor tissues (T) and their adjacent normal colon controls (N) were subjected to immunoblotting analysis with antibodies to USP22, CCNB1 and tubulin. Samples from 10 patients were analyzed. (d) The expression levels of USP22 and CCNB1 in normal human colon tissues (top) and colon cancer tissues (bottom) were determined by immunohistochemistry (IHC) staining with specific antibodies. Representative images are shown. (e) The expression levels of USP22 and CCNB1 in micro tissue array (MTA) slides of paraffin-fixed normal human colon or colon tumor tissue were determined by IHC with specific antibodies, and then scored and analyzed; *P<0.05; **P<0.01; ***P<0.001. NS, no significant difference. (f) The correlation of USP22 and CCNB1 protein expression was analyzed by IHC staining in normal and human colon cancer tissues.