TABLE 1.

Steroid Hormones Evaluated: Limits of Quantification, Precision, and Accuracy of HPLC-MS/MS Measurement

	Steroid Hormone	Short Name	LLOQ (ng/ml)	ULOQ (ng/ml)	Standard	Standards (ng/ml)	Precisiona (%)	Accuracyb (%)
Glucocorticoid	11-Deoxycortisol	11DCORT	5	1000	[2H11]-11DCORT	20, 50, 800	5.0	101.7
	Deoxycorticosterone	DOC	0.5	100	[2H8]-DOC	4, 10, 160	3.7	99.9
	Cortisol	CORTISOL	0.5	100	[2H4]-CORTISOL	20, 50, 800	3.3	99.7
	Corticosterone	CORTICO	0.5	100	[2H8]-CORTICO	20, 50, 800	4.7	100.5
Progestagen	17α-Hydroxyprogesterone	OHPROG	0.2	40	[2H8]-OHPROG	4, 10, 160	4.0	99.6
	17α-Hydroxypregnenolone	OHPREG	5	1000	[2H3]-OHPREG	20, 50, 800	6.7	100
	Progesterone	PROG	0.2	40	[2H9]-PROG	4, 10, 160	3.3	98.1
	Pregnenolone	PREG	2	400	[2H4]-PREG	20, 50, 800	10.0	100.9
Androgen	Dehydroepiandrosterone	DHEA	3	600	[2H5]-DHEA	20, 50, 800	4.0	100.1
	Androstenedione	ANDR	1	200	[2H5]-ANDRO	4, 10, 160	4.7	99.9
	Testosterone	TESTO	0.1	20	[2H5]-TESTO	0.4, 1, 16	5.7	100.7
Estrogen	Estrone	ESTRONE	0.03	6	[2H4]-ESTRO	0.4, 1, 16	5.0	100.4
	Estradiol	ESTRADIOL	0.03	6	[2H5]-ESTRA	0.4, 1, 16	6.3	101.4

ULOQ, upper limit of quantification

^aPrecision was calculated as the %RSD of the standards.

^bAccuracy was calculated as the percent standard recovered.