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. 2016 Mar 28;12(3):e1005918. doi: 10.1371/journal.pgen.1005918

Fig 4. dWnt4 regulates encapsulation of the CB via adherens junction proteins.

Fig 4

(A-B1) Wild type and dWnt4 mutant germaria stained with DE-Cadherin (red) and Zfh1 (green) (white arrow) show perturbed DE-Cadherin expression in dWnt4 mutants. (C-D1) Wild type and dWnt4 mutants stained with β-catenin (red) and Zfh1 (green) (white arrow) display perturbed β-catenin expression in dWnt4 mutants. (E-F) Quantification (n = 5) of DE-Cadherin and β-catenin in the escort cells reveals a downregulation in dWnt4 mutants. (G-H1) dWnt4 heterozygote and dWnt4 mutant germaria stained with GFP (green), Vasa (blue) and 1B1 (red) showing a loss of encapsulation in the dWnt4 mutant. (I-J1) dWnt4 heterozygote and dWnt4 mutant germaria stained with Coracle (red), Vasa (blue) and Zfh1 (green) (white arrow) showing a loss of encapsulation in the dWnt4 mutant. (K-L) Transmission Electron Micrography of dWnt4 heterozygote showing encapsulation (white arrows) and dWnt4 mutant showing loss of encapsulation. CB is highlighted in blue and escort cell is highlighted in green.