Table 2. Enzyme activity rates, and relevant statistics of the P450 activity assays.
| P450 activity rates | 3 weeks | 6 weeks | 9 weeks | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Npc1+/- | Npc1-/- | Npc1+/- | Npc1-/- | Npc1+/- | Npc1-/- | |||||||
| % of Age-Matched Control ± SEM | p—value | % of Age-Matched Control ± SEM | p—value | % of Age-Matched Control ± SEM | p—value | % of Age-Matched Control ± SEM | p—value | % of Age-Matched Control ± SEM | p—value | % of Age-Matched Control ± SEM | p—value | |
| MROD | 89.5 ± 18.0 | 0.0901 | 37.3 ± 4.2 | < 0.0001 | 77.3 ± 17.3 | 0.0005 | 37.3 ± 4.2 | < 0.0001 | 61.4 ± 4.5 | < 0.0001 | 47.9 ± 3.9 | < 0.0001 |
| EROD | 95.0 ± 18.0 | 0.9291 | 42.3 ± 6.4 | < 0.0001 | 64.3 ± 13.9 | 0.0004 | 42.3 ± 6.4 | < 0.0001 | 48.5 ± 4.1 | < 0.0001 | 49.2 ± 3.1 | < 0.0001 |
| PROD | 92.5 ± 6.6 | > 0.9999 | 61.8 ± 7.0 | < 0.0001 | 66.1 ± 7.1 | < 0.0001 | 61.8 ± 7.0 | < 0.0001 | 86.3 ± 2.6 | 0.0001 | 67.6 ± 6.2 | < 0.0001 |
| BROD | 96.2 ± 9.3 | > 0.9999 | 36.1 ± 7.6 | < 0.0001 | 53.1 ± 11.2 | 0.0004 | 36.1 ± 7.6 | < 0.0001 | 51.9 ± 5.7 | < 0.0001 | 41.9 ± 5.4 | < 0.0001 |
Enzyme activity rates of MROD, EROD, PROD and BROD at 3, 6 and 9 weeks of age in Npc1+/- and Npc1-/- mice as compared with wild type age-matched control. Data are presented as mean ± SEM, n = 6, calculated using two-tailed unpaired nonparametric Mann-Whitney test. All data was standardized to Npc1+/+ mouse data with enzyme activity rates of 100%.