FIG. 5.

Inhibitors of classical and nonclassical T signaling disrupt spermatogenesis in testis explants. A) Testis explants from 35-day-old wild-type rats were cultured with no adenovirus or adenovirus constructs expressing S1s, S3, H-K-AR122, or S3 + H-K-AR122. After 6 days, RNA was extracted from the explants and reverse transcription (RT) assays performed followed by qPCR quantitation of relative Rhox5 RNA levels. Data shown are representative of assays from three isolations of explants. B–D) Testis explants from 35-day-old tfm rats were infected with no adenovirus or adenovirus constructs expressing ARwt (WT), nonclassical pathway activator (ARC562G), classical pathway activator (ARΔ372-385), or both activators (ARC562G + ARΔ372-385). The relative RNA levels and SEM for Gapdh (B, n = 3), Zbtb16 (C, n = 3), and c-Kit (D, n = 5) were assayed using RNA extracted from the explants 6 days after adenovirus infection and made relative to no adenovirus (=1). ANOVA statistical analysis with Newman-Keuls PLSD was used. Values with different lowercase letters differ significantly (P < 0.05).