WIN 18 446 treatment increases recombination rate. Animals (n = 4) were treated with WIN 18 446 for 12 days. Meiotic spreads and IHC were performed to determine recombination rates. A and B) Representative images of cells from control and WIN 18 446-treated animals, respectively. Purple and green staining are representative of SYCP3 and MLH1, respectively. e0, e1, e2, and e3 denote the percentage of chromosomes containing 0, 1, 2, and 3 exchanges, represented by MLH1. C) A Student t-test was used to determine if there was a statistically significant difference in recombination rate between control and WIN 18 446-treated animals (*P < 0.05). Bar = 10 μm.