Effect of prenatal testosterone exposure on insulin responses following oral glucose tolerance test in intact (A) and orchiectomized (B) control and prenatally TP-exposed males and on insulin AUC (C) generated from the glucose tolerance test. Blood samples were collected at 0, 30, 60, 90, 120, 180, and 240 min following oral glucose (2 g/kg) administration. The overall plasma glucose levels are expressed as the AUC calculated by the trapezoidal method. Bars with different superscript letters differ significantly (P < 0.05). All data are expressed as the mean ± SEM (n = 6–8 animals in each group). OCX, orchiectomy.