FIG. 9.

Effect of prenatal testosterone exposure on β-cell area. Pancreatic sections from intact and orchiectomized control and prenatally TP-exposed males were stained with anti-insulin antibody. A) Representative immunofluorescence images of insulin. Original magnification ×40. B and C) Insulin staining intensity (green fluorescence) per nuclei (blue fluorescence) (B) and β-cell area (C) relative to total pancreas area were quantified from pancreatic sections. Four clusters per pancreatic section and three pancreatic sections per animal were examined. Values are presented as the mean ± SEM (n = 5 animals in each group). Different superscript letters indicate significant differences (P < 0.05).